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KMID : 0363620100310010138
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 1 p.138 ~ p.152
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Effect of Yikgeebohyul-tang (Yiqib?xue-t?ng) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice
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Hong Jung-Ae
Song Mi-Yeon
Choi In-Hwa
Sohn Nak-Won
Chung Seok-Hee
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Abstract
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Objective: Yikgeebohyul-tang (Yiqib?xue-t?ng) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yiqib?xue-t?ng) on hair regrowth and cytokine changes in a shaving model of C57BL6 mice.
Method: Five-week-old mice were acclimated for 1 week at a temperature between 21-23℃E, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yiqib?xue-t?ng) extract (sample) in 18 female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF-β1 and EGF were also determined using immunohistochemistry. In addition to that, IFN-γ, IL-4 and IL-10 were determined in serum.
Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF-β1 was not revealed in either control orsample group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN-γ was significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance.
Conclusion: These results suggest that Yikgeebohyul-tang (Yiqib?xue-t?ng) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN-γ, and an increase of serum IL-4.
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KEYWORD
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Alopecia, C57BL/6 mice, Yikgeebohyul-tang, hair regrowth
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